First Record of Phlebotomus (Larroussius) perfiliewi (Diptera: Psychodidae), Vector of Leishmania infantum and Phleboviruses, in Spain

Phlebotomine sand flies are vectors of several diseases importance for public health, including leishmaniosis, bartonellosis, and fly fevers. An entomological survey on blood-feeding Diptera was conducted in June–November 2020–2021 to know the diversity insect Mallorca (Balearic Islands, Spain). Among collected, Phlebotomus (Larroussius) perfiliewi Parrot, 1930 found being first record this species Spain. s.l. is one main Leishmania infantum Mediterranean Basin Central Asia. The identification confirmed by both morphological features DNA barcoding. Phylogenetic analyses showed that specimens captured were Ph. s.s. (99.85–100% homologues from Italy Algeria specimens), with a sequence divergence 0.17%. cytochrome c oxidase subunit I gene clearly separates three make up complex. In addition, we also provide brief discussion about their remarks, phylogenetic relationships, vector status.